Task Effects of Alphalipoic Acid on Irradiation Induced Cognitive Deficits (Postdoctoral Fellowship) (Completed)
Last Published:  07/31/19 10:05:33 AM (Central)
Short Title: Effects of Alpha Lipotic Acid on Mice
Responsible HRP Element: Space Radiation
Collaborating Org(s):
National Space Biomedical Research Institute (NSBRI)
Other:
Funding Status: Completed - Task completed and produced a deliverable
Procurement Mechanism(s):
Solicited
Aims:
  1. To determine the short-term effects of 56Fe radiation irradiation on hippocampal function and assess whether these effects are sex-dependent. Mice will be irradiated at 0, 0.1, 0.2, or 0.5 Gy and behaviorally tested 1-2 weeks following radiation.
  2. To determine whether the severity of the cognitive effects are associated with reduced choline acetyltransferase (ChAT) immunoreactive cell bodies in the medial septum and nucleus basalis and ChAT immunoreactive fibers in the hippocampus and cortex. Following behavioral testing, ChAT cell number and fiber density will be determined using immunohistochemistry and measures of ROS by western blot.
  3. To determine the ability of the dietary supplement lipoic acid (LA) to antagonize the effects of 56Fe irradiation on hippocampus-dependent cognitive function, measures of ROS, and ChAT immunoreactive cell bodies in the medial septum and nucleus basalis and ChAT immunoreactive fibers in the hippocampus and cortex. Mice will be sham-irradiated or irradiated at a dose causing maximal cognitive injury in Aim 1 and receive regular diet or LA-containing diet starting one week prior to behavioral testing. Brains will be processed as in Aim 2.
Resources (None Listed)
Mappings