Funding Status:
Completed - Task completed and produced a deliverable
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Procurement Mechanism(s):
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Aims:
PART 1) HIGH DIETARY HEME IRON COMBINED WITH IRRADIATION PROMOTES A PROATHEROGENIC ENDOTHELIAL CELL PHENOTYPE DUE TO INCREASED OXIDATIVE STRESS
SPECIFIC AIMS
Aim 1: To determine the short-term consequences of the independent and combined effects of exposure to gamma radiation and elevated body iron stores on measures of endothelial function. We hypothesize that animals that have high body iron stores and are exposed to gamma radiation will show a greater reduction in endothelial dependent nitric oxide/cGMP production than animals that have only 1 of those treatments (either high iron stores or exposure to gamma radiation).
Aim 2: Identify and compare the effects of gamma radiation and elevated body iron stores on the genetic and epigenetic regulation of proteins associated with endothelial cell barrier function. We hypothesize that modifications of genetic control and posttranslational expression of proteins associated with endothelial cell barrier function will be differentially altered in rats with high body iron stores and exposed to gamma radiation compared to rats with only 1 type of treatment.
PART 2) OXIDATIVE MECHANISMS OF MYOCARDIAL TISSUE REMODELING IN A MODEL OF HIGH DIETARY HEME IRON EXPOSED TO RADIATION
Aim 1: To determine the short-term consequences of the independent and combined effects of gamma radiation and elevated body iron stores on measures of cardiac structure. We hypothesize that modifications to cardiac structure and function will be greater in rats with high body iron stores and exposed to gamma radiation than in rats that have only 1 of those treatments.
Aim 2: Identify and compare the effects of gamma radiation and elevated body iron stores on the genetic and epigenetic regulation of proteins associated with cardiac structure and function. We hypothesize that modifications of genetic control and posttranslational expression of proteins associated with cardiac contractile function will be differentially altered in rats with high body iron stores and exposed to gamma radiation compared to rats with only 1 type of treatment.