Task The Effect of High LET Radiation on Differentiation and Tumorigenesis in the Human Hematopoietic System: Modeling in vitro and in vivo for Risk Assessment (Completed)
Last Published:  03/26/21 03:34:00 PM (Central)
Short Title: Hematopoietic System
Responsible HRP Element: Space Radiation
Collaborating Org(s):
Funding Status: Completed - Task completed and produced a deliverable
Procurement Mechanism(s):
The study is based on a stepwise enrichment strategy the first stage of which will be to irradiate mice engrafted with human hematopoietic system followed 3 months later by analysis of human stem and progenitor cells from mouse bone marrow for chromosomal damage and developmental abnormalities. The goal in this initial stage will be to identify human pre-leukemic stem cells and progenitor cells which exhibit altered karyotypes and abnormal differentiation patterns. The second stage involves engraftment of human hematopoietic stem cells with known abnormalities in secondary mouse hosts for further tumor developmental studies. This secondary host transplantation strategy which is commonly used in human tumor engraftment studies will allow the enrichment of the engrafted mouse cohorts for potentially tumorigenic carriers and permit elimination of the mice with normal karyotypes which usually don’t develop tumors.
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