Task Dry Saliva Validation (Lab)
Last Published:  07/30/21 01:05:34 PM (Central)
Short Title: Dry Saliva Validation
Responsible HRP Element: Human Health Countermeasures
Collaborating Org(s):
Funding Status: Active - Currently funded and in progress
Procurement Mechanism(s):
HHC Unsolicited Task
TBD
Aims:
1.To identify the best paper/filters and pretreatment requirements for successful saliva sample
collection, drying and reconstitution/recovery of saliva analytes. Various substrates and
methods for reconstitution will be evaluated. While recovery of all analytes may not be
possible, we will identify a suite of assays compatible with dry saliva storage that can support
Gateway missions.
-Cytokines (IL-6, TNFa, IL-12, IL-8, etc)
-antimicrobial proteins (defensins HNP 1-3, lysozyme, LL37)
-latent virus DNA (VZV, EBV, HSV-1)
-Virus DNA detection (TTV)
-Immune/Inflammatory markers (CRP, IgA, IgG, IgM)

2. To establish the long term stability (durations relevant for Gateway collection and return) of each detectable analyte under dry storage conditions
Mappings