Task Development and use of human 3-Dimensional tissue culture models for the study of space radiation effects on the central nervous system (Completed)
Last Published:  07/31/19 10:05:33 AM (Central)
Short Title: 3D CNS
Responsible HRP Element: Space Radiation
Collaborating Org(s):
Other:
Funding Status: Completed - Task completed and produced a deliverable
Procurement Mechanism(s):
Solicited
Aims:
The primary objective of the research in this proposal is to increase our understanding of the effects of space radiation on the human central nervous system with an emphasis on neurodegeneration. We are using a relatively new approach to this question by using available human stem cells to create 3-dimensional tissue models. These can represent human neural tissue more closely than 2-dimensional monolayers. Human cells of three of the main types in the brain will be cultured and differentiated in 3-D matrix gels and used to determine the effects of space radiation by assaying for specific endpoints related to degeneration in neurons. This is achieved by live and fixed stain 3-D imaging together with assays for secreted proteins. 3-D cultures are grown in gels as monocultures and as a multi-cell ‘neurovascular unit’ built on an existing model of human vessels. Such a model can complement animal and cell studies to produce new and more complete data on the effects of space radiation on the human nervous system.
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