Task Effects of Alphalipoic Acid on Irradiation Induced Cognitive Deficits (Postdoctoral Fellowship) (Completed)
Last Published:  07/30/20 02:45:15 PM (Central)
Short Title: Effects of Alpha Lipotic Acid on Mice
Responsible HRP Element: Space Radiation
Collaborating Org(s):
National Space Biomedical Research Institute (NSBRI)
Other:
Funding Status: Completed - Task completed and produced a deliverable
Procurement Mechanism(s):
Solicited
Aims:
  1. To determine the short-term effects of 56Fe radiation irradiation on hippocampal function and assess whether these effects are sex-dependent. Mice will be irradiated at 0, 0.1, 0.2, or 0.5 Gy and behaviorally tested 1-2 weeks following radiation.
  2. To determine whether the severity of the cognitive effects are associated with reduced choline acetyltransferase (ChAT) immunoreactive cell bodies in the medial septum and nucleus basalis and ChAT immunoreactive fibers in the hippocampus and cortex. Following behavioral testing, ChAT cell number and fiber density will be determined using immunohistochemistry and measures of ROS by western blot.
  3. To determine the ability of the dietary supplement lipoic acid (LA) to antagonize the effects of 56Fe irradiation on hippocampus-dependent cognitive function, measures of ROS, and ChAT immunoreactive cell bodies in the medial septum and nucleus basalis and ChAT immunoreactive fibers in the hippocampus and cortex. Mice will be sham-irradiated or irradiated at a dose causing maximal cognitive injury in Aim 1 and receive regular diet or LA-containing diet starting one week prior to behavioral testing. Brains will be processed as in Aim 2.
Resources (None Listed)
Mappings
RiskRisk of Adverse Cognitive or Behavioral Conditions and Psychiatric Disorders
GapBMed-102: Given exposures to spaceflight hazards (space radiation, isolation), how do we identify individual susceptibility, monitor molecular/biomarkers and acceptable thresholds, and validate behavioral health and CNS/neurological/neuropsychological performance measures and domains of relevance to exploration class missions?
GapBMed-103: What are the validated, efficacious treatments (individual or Team-based) and/or countermeasures to prevent adverse behavioral conditions, CNS/neurological, and/or psychiatric disorders caused by either single and/or integrated exposures to spaceflight hazards during exploration class missions?
GapBMed-104: Given the potentially negative spaceflight associated CNS changes and behavioral experiences of stressors during long-duration missions (e.g., isolation, confinement, reduced sensory stimulation, altered gravity, space radiation), what are validated modifications to habitat/vehicle to mitigate stressors impacting on CNS / cognition / behavioral health?
GapBMed-105: Given the potentially negative spaceflight associated CNS/cognitive changes and behavioral experiences of stressors during long-duration missions (e.g., isolation, confinement, reduced sensory stimulation, altered gravity, space radiation), what are validated medical or dietary countermeasures to mitigate stressors impacting on CNS / cognition / behavioral health?
GapBMed-107: What are the long-term changes and risks to astronaut health post-mission that, when using a continuity of care model, helps retrospectively identify and understand individual susceptibility (e.g., hereditary, dose, thresholds) to mitigate adverse CNS, cognitive, and behavioral health changes resulting from long-duration exploration missions, promoting the behavioral health of current and future crews?
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