Task Induction of Early Stages of Osteoarthritis After Exposure to Microgravity (NSBRI Postdoctoral Fellowship) (Completed)
Last Published:  07/29/22 01:33:24 PM (Central)
Short Title: Early Arthritis
Responsible HRP Element: Human Health Countermeasures
Collaborating Org(s):
National Space Biomedical Research Institute (NSBRI)
Funding Status: Completed - Task completed and produced a deliverable
Procurement Mechanism(s):

This project’s goal is to investigate the effects of microgravity on cartilage homeostasis by exposing two chondrocyte cell lines to a modeled microgravity environment using a rotating wall vessel bioreactor. Cells exposed to microgravity will be compared to cells incubated under normal gravitational conditions as a control, as well as an arthritic-like cell model induced by treating chondrocytes with pro-inflammatory cytokines such as IL-1B and oncostatin M (OSM). Disruptions in cell-matrix interactions, changes in cytoskeletal morphology and gene up-regulation will be evaluated to determine changes in chondrocyte metabolism.