Task Evaluation of Host-Pathogen Interactions During Exposure to Microgravity Analogues -- ASU Grant (Completed)
Last Published:  07/30/21 01:05:34 PM (Central)
Short Title: Host Pathogen
Responsible HRP Element: Space Human Factors and Habitability
Collaborating Org(s):
Funding Status: Completed - Task completed and produced a deliverable
Procurement Mechanism(s):
Solicited
Aims:
The aim of this study is to determine if the conditions experienced by both the host and pathogen during co-culture in a spaceflight analogues (NASA designed rotating wall vessel (RWV) bioreactors, which randomize the gravity vector, simulate many of the mechanistic effects of microgravity on biological systems) will alter their interactions and thus the risk for infectious disease. The work examines the effect of modeled microgravity (MMG) on the interactions between host (3-D models of human cell cultures) and relevant pathogens (bacterial and viral) when both are simultaneously exposed to MMG. Endpoints to be assayed include adherence, invasion, and intracellular survival profiles, tissue pathology, innate immune responses, and select genomic/proteomic responses of the infected host.


Key findings: 3-D models respond to infection with bacterial and viral pathogens in ways that reflect the infection process in vivo.  Work with the models facilitated identification of novel host biosignatures in response to infection (including Salmonella sp, Norwalk virus, and Pseudomonas aeruginosa), and allowed study of infectious disease agents that lack suitable cell culture and animal models.

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