Task Charged Particle Effects on the Ovary (Completed)
Last Published:  11/23/20 11:55:12 AM (Central)
Short Title: SR Ovary
Responsible HRP Element: Space Radiation
Collaborating Org(s):
Funding Status: Completed - Task completed and produced a deliverable
Procurement Mechanism(s):
Solicited
Aims:
We propose to test the effects of low dose charged particle radiation (low LET oxygen and high LET iron ions), typical of exposures in space, on ovarian follicles (the functional unit of the ovary) and on ovarian carcinogenesis in adult female mice. We will use histomorphometric methods to quantify the effects of charged particles on numbers of ovarian follicles and ovarian tumor multiplicity and size. We will use in situ methods to assess apoptosis and proliferation, measure endpoints of ovarian oxidative stress, and conduct ovarian transcriptomic analyses to identify signaling pathways that are perturbed by charged particle radiation and which may play a role in ovarian tumorigenesis. Our previous studies have shown that oxidative stress plays a role in and the antioxidant glutathione is protective against radiation- and chemical-induced damage to ovarian cells and ovarian carcinogenesis. We will therefore also examine whether antioxidant supplementation is protective against and whether genetic deficiency in glutathione increases susceptibility to the adverse ovarian effects of charged particle radiation.
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