Task Acute and long term outcomes of simulated deep space radiation exposure on latent viral CNS infection & CNS pathology (Completed)
Last Published:  07/30/20 02:45:15 PM (Central)
Short Title: SR CNS Infection & Pathology
Responsible HRP Element: Space Radiation
Collaborating Org(s):
Funding Status: Completed - Task completed and produced a deliverable
Procurement Mechanism(s):
In this proposal, the effects of simulated deep space radiation on the development of CNS pathology (reactive lesions) and the impact of latent and induced in vivo reactivation of HSV infection on the development of CNS pathology will be determined together with the role that human ApoE alleles play in these processes. We will employ our well characterized mouse model of HSV latency and in vivo reactivation in combination with quantitative measures of reactive lesions in the CNS to determine the effect of simulated deep space radiation on HSV mediated CNS damage. The acute stage of infection will be monitored using our standard approaches including quantitative evaluation of infectious virus titers. Latent infection in the trigeminal ganglia and brain will be quantified at 30-40 days postinfection (pi) using our quantitative real time PCR assay for the viral genome. Spontaneous and induced exit from latency will be quantified at the single neuron level using our whole TG immunohistochemical assay. This approach permits us to identify even a single positive neuron in groups of ganglia. Viral reactivation (production of infectious virus) will be evaluated using our standard assay. Reactivation will be induced using our well-established hyperthermic stress procedure (temperature of the mice is raised to 42.5 C for 10 minutes).
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