Description:
This proposal will produce two public resources and one novel data set. First, provide an
updated set of code for existing bioinformatics tools in genome annotation and epigenetic analysis
(HUGE-seq, methylKit, eDMR, r-make). Second, connect these existing tools with other new
tools in epigenetics research (methclone and ENCODE-based MongoDB) and place these into a web
interface for NASA grantees and other scientists. Finally, use these tools to highlight the sites
and regions of the astronaut genomes that are epigenetically impacted by space travel, and then create single-cell chromatin state data (ATAC-seq) to validate these areas and measure the proportion of cells impacted by these changes – the first ever of such data to be created.