Task Variation in CNS damage signaling and blood sentinels of neuropathology after exposure to space radiation.
Last Published:  07/31/19 10:05:33 AM (Central)
Short Title: CBS CNS Damage Signaling Neuropathology (Wyrobek)
Responsible HRP Element: Human Factors and Behavioral Performance
Collaborating Org(s):
Human Health Countermeasures (HHC) Element
Other:
Space Radiation (SR) Element
Funding Status: Active - Currently funded and in progress
Procurement Mechanism(s):
NIPR
Aims:
Aim 1. Characterize the persistence of radiation-induced molecular abnormalities in cortex and hippocampus after low-dose exposures to 56Fe particles, and compare the predictions for CNS tissue damage and late-onset neuropathologies in similarly irradiated mice and rats.
Aim 1a: Use archived CNS tissue from SD rats collected at 4 and 9 months after low-dose exposures, and generate new transcriptomics (3’TAGseq) and new metabolomics profiles (untargeted metabolism and targeted complex lipids) from cortex and hippocampus. These data will be integrated with prior proteomics profiles from hippocampus at 9 months after exposure to build a predictive model of CNS tissue damage and risks for neuropathology, with emphasis on vascular and immune abnormalities.
Aim 1b: Compare molecular responses in cortex and hippocampus of mice and rats at 9 months after exposure to evaluate cross- species consistency. The predictive model will be built using integrating bioinformatics and biostatistical approaches.
AIM 2. Identify persistent bio-effect markers in peripheral blood and CSF that correlate with molecular damage in CNS vascular or immune functions.
Aim2A will investigate the metabolomics profiles of archived CSF and archived peripheral blood plasma for the same animals. 
AIM 2B will determine whether the blood metabolities associated with CNS tissue damage correlate with individual variation in radiation-susceptibility for anxiety as measured in the elevated plus maze.
Resources (None Listed)
Mappings
RiskRisk of Acute (In-flight) and Late Central Nervous System Effects from Radiation Exposure
You are here!TaskVariation in CNS damage signaling and blood sentinels of neuropathology after exposure to space radiation.

RiskRisk of Adverse Cognitive or Behavioral Conditions and Psychiatric Disorders
You are here!TaskVariation in CNS damage signaling and blood sentinels of neuropathology after exposure to space radiation.

RiskRisk of Adverse Health Event Due to Altered Immune Response
You are here!TaskVariation in CNS damage signaling and blood sentinels of neuropathology after exposure to space radiation.

RiskRisk of Cardiovascular Disease and Other Degenerative Tissue Effects From Radiation Exposure and Secondary Spaceflight Stressors
You are here!TaskVariation in CNS damage signaling and blood sentinels of neuropathology after exposure to space radiation.