Task Astrocytes as key mediators of central nervous system responses to space radiation
Last Published:  07/29/22 01:33:24 PM (Central)
Short Title: CBS Astrocyte Mediator (Cekanaviciute)
Responsible HRP Element: Human Factors and Behavioral Performance
Collaborating Org(s):
Human Health Countermeasures (HHC) Element
Space Radiation (SR) Element
Funding Status: Active - Currently funded and in progress
Procurement Mechanism(s):
Specific Aim 1. Define human astrocyte responses to ionizing radiation. We will map
the dose response and time course of astrocyte responses to X-rays, and 0.5Gy 5-ion simulated
GCRs to identify their relative biological effectiveness (RBE). We will quantify astrocyte gene
expression and correlate it with physiological outcomes: cytokine secretion, cell death and
oxidative stress, to define whether irradiated astrocytes adopt A1 or A2 phenotypes.

Specific Aim 2. Investigate how astrocytes regulate neuronal damage in response to
simulated GCRs. We will utilize 3D CNS TOC models (Mimetas OrganoPlates, Figures 2, 3.)
to define 0.5Gy 5-ion simulated GCR-induced neuronal damage and evaluate the contribution of
astrocytes to regulating it.

Specific Aim 3. Examine whether inducing A1/A2 astrocyte phenotypes could serve
as a countermeasure to simulated GCRs. We will induce TOC astrocyte differentiation into
either A1 or A2 phenotype, and examine whether they exacerbate or inhibit neuronal damage
caused by 0.5Gy simulated GCRs.

Resources (None Listed)