Task Animal radiation study (Radiation Pilot-Westby, Completed) (Completed)
Last Published:  07/30/20 02:45:15 PM (Central)
Short Title: Radiation Pilot
Responsible HRP Element: Human Health Countermeasures
Collaborating Org(s):
Human Health Countermeasures (HHC) Element
Funding Status: Completed - Task completed and produced a deliverable
Procurement Mechanism(s):
Solicited
Aims:
Empirical data from studies using animals indicate that moderate to high doses of x-ray and gamma radiation indeed contribute to accelerated atherosclerosis and impaired nitric oxide-mediated vasodilation. However, there are reported observations suggesting that exposure to low-dose radiation may impart a protective effect if it precedes a larger single dose exposure. To date, few studies have examined whether exposure to low-dose radiation provides a means for mitigating the effects of an acute high-dose exposure. Therefore, using this radiation dosing paradigm (low-dose followed by a single high-dose radiation) in a mouse model, we will determine if the tissue that forms the cardiovascular system (heart and arteries) are protected from the damaging effects of high doses of gamma radiation when previously exposed to a low-dose of radiation.

Specific Aims:
  1. to measure inter and intracellular levels of oxidative (glutathione and SOD activity) and inflammatory stress (IL-6 and TNF-a) in the 3 different radiation treatment different groups (plus control).
  2. investigate potential tissue changes by measuring cellular markers of apoptosis from tissues that form the heart and descending aortic artery.
Methods:  In the preliminary experiment, the mice will be exposed to a low dose of 0.05 Gy, followed by a high dose of 6 Gy 24 hr later. The mice will be sacrificed three days after the high dose exposure. More time points in both the time interval between the priming and the challenging doses, and the time to sacrifice the animal after the challenging dose will be added in the comprehensive experiment.
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