Task High Dimensional Biology to Understand the Functional Response of Salmonella to Long-Term Multigenerational Growth in the Chronic Stress of Microgravity
Last Published:  11/23/20 11:55:12 AM (Central)
Short Title: SB EVOLVES
Responsible HRP Element: Human Health Countermeasures
Collaborating Org(s):
Space Biology Program
Funding Status: Active - Currently funded and in progress
Procurement Mechanism(s):
Solicited
Aims:
This study will evaluate if long-term multigenerational culture of Salmonella enterica serovar Typhimurium (S. Typhimurium) in the spaceflight environment will result in stress-induced mutations and genomic instability as reflected in changes in the genomic, epigenetic, transcriptomic, proteomic and select phenotypic characteristics (including biofilm production, antibiotic resistance and virulence) that will reflect a central role for Hfq as a global regulator of these heritable responses.

Aim 1: Characterize the impact of long-term, multigenerational growth during spaceflight on the genotype of both an S. Typhimurium wild type and hfq mutant strain.
Aim 2: Characterize the impact of long-term, multigenerational growth during spaceflight on the transcriptomic profiles of both an S. Typhimurium wild type and hfq mutant strain.
Aim 3: Characterize the impact of long-term, multigenerational growth during spaceflight on the proteomic profile of both an S. Typhimurium wild type and hfq mutant strain.
Aim 4: Characterize the impact of long-term, multigenerational growth during spaceflight on the virulence and pathogenesis-related characteristics of both an S. Typhimurium wild type and hfq mutant strain.

Mappings
RiskRisk of Adverse Health Effects Due to Host-Microorganism Interactions
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