Funding Status:
Active - Currently funded and in progress
|
Procurement Mechanism(s):
|
|
Aims:
This study will evaluate if long-term multigenerational culture of
Salmonella enterica serovar Typhimurium (S. Typhimurium) in the spaceflight environment will result in stress-induced mutations and genomic instability as reflected in changes in the genomic, epigenetic, transcriptomic, proteomic and select phenotypic characteristics (including biofilm production, antibiotic resistance and virulence) that will reflect a central role for Hfq as a global regulator of these heritable responses.
Aim 1: Characterize the impact of long-term, multigenerational growth during spaceflight on the genotype of both an S. Typhimurium wild type and hfq mutant strain.
Aim 2: Characterize the impact of long-term, multigenerational growth during spaceflight on the transcriptomic profiles of both an S. Typhimurium wild type and hfq mutant strain.
Aim 3: Characterize the impact of long-term, multigenerational growth during spaceflight on the proteomic profile of both an S. Typhimurium wild type and hfq mutant strain.
Aim 4: Characterize the impact of long-term, multigenerational growth during spaceflight on the virulence and pathogenesis-related characteristics of both an S. Typhimurium wild type and hfq mutant strain.