Task Early Markers of Space-Radiation Induced Human Cataractogenesis (Completed)
Last Published:  07/30/20 02:45:15 PM (Central)
Short Title: Human Cataract
Responsible HRP Element: Space Radiation
Collaborating Org(s):
Funding Status: Completed - Task completed and produced a deliverable
Procurement Mechanism(s):
Solicited
Aims:
In order to assess the time course of the expression and the natural history of the developing lesion, two experimental models of the lens are proposed in this application. Differentiating human lens cells in vitro will be used to extend the investigation of molecular mechanisms of action that follow particle radiation induced changes in key marker proteins using beams defined by NASA's operational parameters, eg. low doses and dose-rates of protons or heavy ions. Mice will be irradiated and early lenticular changes associated with molecular aggregation will be followed using an exquisitely sensitive dynamic light scattering method to map the unique signatures of particle radiation-induced lens opacification. Information gathered from these measurements will be used to determine when the lenses will be harvested for protein identification. The results of the proposed research will improve mechanistic understanding underlying variability of dose-rate dependencies for cataract formation, and reduce the uncertainties in cataract risk assessment.
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