Task The Role of Gap-Junction Communication and Oxidative Metabolism in the Biological Effects of Space Radiation (Completed)
Last Published:  11/23/20 11:55:12 AM (Central)
Short Title: Biological SR
Responsible HRP Element: Space Radiation
Collaborating Org(s):
Funding Status: Completed - Task completed and produced a deliverable
Procurement Mechanism(s):
Solicited
Aims:
In Aim 1, we propose to define the aspect(s) of junctional communication leading to proton- and high charge/high energy (HZE) particle-induced toxic effects. Specifically, we will examine the role of different connexin proteins in propagating death-inducing (growth delay, apoptosis) or pro-survival effects (proliferative capacity) among irradiated cells and also between irradiated and non-irradiated cells. Aim 2 is to identify specific connexin channel permeabilities that correlate with intercellular propagation of toxic or protective effects in cultures exposed to HZE particles or protons. An emphasis will be to determine the nature of molecules communicated among irradiated cells and between irradiated and non-irradiated cells. In Aim 3, we will evaluate mitochondrial function in cells exposed to low fluences of high energy charged particles. Specifically, we will examine mitochondrial protein import, mitochondrial protein synthesis, assembly of large protein complexes including respiratory complexes, and oxidative damage to mitochondrial proteins and DNA. We will also evaluate the persistence of oxidative effects in the progeny of exposed cells and correlate the observed results with the overall cellular redox environment.
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