Task Systematic Identification of Genes and Signal Transduction Pathways Involved in Radio-Adaptive Response (Completed)
Last Published:  11/23/20 11:55:12 AM (Central)
Short Title: Radio-adaptive Response
Responsible HRP Element: Space Radiation
Collaborating Org(s):
Department of Energy (DoE)
Funding Status: Completed - Task completed and produced a deliverable
Procurement Mechanism(s):
Directed
Aims:
  1. We will expose C57BL/6 mice to Cs-137 gamma rays at a priming low dose followed by a challenging high dose, and investigate the adaptive response effects by measuring the endpoints of (1) survival of the animal, (2) chromosome aberrations in the lymphocytes, bone marrow, and spleen, and (3) ROS induction in the serum.
  2. Using the real-time PCR array technique, we will measure gene expression changes in the BFO, spleen, brain and intestine of the mice after exposures to the priming dose and again to the challenging dose. These gene expression changes will be compared to those in the mice that are exposed to the challenging dose alone. Genes involved in stress response, immune functions and DNA repairs will be the focus groups. Selected gene expression changes will be verified with a protein assay. We will apply bioinformatics tools to identify signaling pathways responsible for radio-adaptation.
  3. For one or two selected genes that are identified as a major player in radio-adaptation from Specific Aim 2 above, we will investigate the radio-adaptive effect in knock-out mice for the selected gene by measuring the endpoints as described in Specific Aim 1. Gene expressions in the knock-out mice after exposures will also be measured as described in Specific Aim 2.
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